CST, 13817S, KIF3B Antibody

Polyclonal Antibody for studying KIF3B. Validated for Western Blotting. Highly specific and rigorously validated in-house, KIF3B Antibody (CST #13817) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity KIF3B Antibody recognizes endogenous levels of total KIF3B protein. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human KIF3B protein. Antibodies are purified by protein A and peptide affinity chromatography. Background Kinesin superfamily proteins (KIFs) are molecular motors that drive directional, microtubule-dependent intracellular transport of membrane-bound organelles and other macromolecules (e.g., proteins, nucleic acids). The intracellular transport functions of KIFs are fundamentally important for a variety of cellular functions, including mitotic and meiotic division, motility/migration, hormone and neurotransmitter release, and differentiation (1-4). Disruptions to KIF-mediated intracellular transport have been linked with a variety of pathologies, ranging from tumorigenesis to defects in higher order brain function such as learning and memory (4-6). KIF3B binds the related kinesin family member 3A (KIF3A) and kinesin-associated protein 3 (KAP3) to form kinesin-2, an ATP-dependent heterotrimeric motor protein complex that uses plus-end-directed microtubule sliding activity to drive anterograde organelle transport (7). Studies in the zebrafish model suggest that KIF3B is required for the development and function of cilia in multiple cell types (8). Research studies show that KIF3B protein is upregulated in human hepatocellular carcinoma and that its expression is associated with poor prognosis in this cancer type (9). Alternate Names FLA8; HH0048; KIAA0359; KIF3B; kinesin family member 3B; Kinesin-like protein KIF3B; Kinesin-like protein KIF3B, N-terminally processed; KLP-11; Microtubule plus end-directed kinesin motor 3B; RP89 Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 85 SOURCE: Rabbit