CST, 13826S, ERR-alpha (E1G1J) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying ERR1. Validated for Western Blotting,Chromatin IP,Chromatin IP-seq. Available in 2 sizes. Highly specific and rigorously validated in-house, ERR-alpha (E1G1J) Rabbit Monoclonal Antibody (CST #13826) is ready to ship. Product Usage Information For optimal ChIP and ChIP-seq results, use 10 μl of antibody and 10 μg of chromatin (approximately 4 x 10 6 cells) per IP. This antibody has been validated using SimpleChIP ® Enzymatic Chromatin IP Kits. Western Blotting: 1:1000 Chromatin IP: 1:50 Chromatin IP-seq: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Chromatin IP, Chromatin IP-seq Specificity / Sensitivity ERR-alpha (E1G1J) Rabbit Monoclonal Antibody recognizes endogenous levels of total ERRα protein. This antibody does not cross-react with ERR family members ERRβ and ERRγ, and does not cross-react with either ERα or ERβ. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human ERRα protein. Background The estrogen-related receptor (ERR) subfamily of orphan nuclear receptors include three protein receptors, ERRα/NR3B1, ERRβ/NR3B2, and ERRγ/NR3B3, that have yet to be associated with natural ligands. PGC-1 coactivators regulate ERR transcription activation ability and receptor-induced transcription of genes involved in lipid metabolism, glucose metabolism, and mitochondrial biogenesis (1). Estrogen-related receptor α (ERRα/NR3B1) is an orphan nuclear receptor that controls transcription of genes involved in fatty acid oxidation, glucose metabolism, and mitochondrial biogenesis (1,2). The receptor protein contains a non-conserved amino terminal domain (NTD), a central zinc finger DNA binding domain, and a ligand-binding domain. The carboxy-terminal AF2 helix motif of ERRα contains binding sites for nuclear receptor coactivators PGC-1α and PGC-1β (3-5). Research studies demonstrate that ERRα transcriptional activity is regulated through phosphorylation and sumoylation within the NTD (6). ERRα is ubiquitously expressed, with strong expression observed in heart, kidneys, skeletal muscle, and other high metabolic demand tissues (2). Additional studies indicate that ERRα is coexpressed in breast tumors with unfavorable biomarkers (7). The pharmacologic inhibition of ERRα activity in breast cancer might serve as a valuable therapeutic approach (8,9). Alternate Names ERR-alpha; ERR1; ERRa; ERRalpha; ESRL1; ESRRA; Estrogen receptor-like 1; estrogen related receptor alpha; estrogen-related nuclear receptor alpha; Estrogen-related receptor alpha; NR3B1; Nuclear receptor subfamily 3 group B member 1; Steroid hormone receptor ERR1 Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 50 Source/Isotype: Rabbit IgG