CST, 13829T, NLRX1 (D4M3Z) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying NOD5. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, NLRX1 (D4M3Z) Rabbit Monoclonal Antibody (CST #13829) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity NLRX1 (D4M3Z) Rabbit Monoclonal Antibody recognizes endogenous levels of total NLRX1 protein. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val437 of human NLRX1 protein. Background The nucleotide-binding oligomerization domain (NOD)-like receptor (NLR) family of proteins is a diverse family of cytoplasmic innate immune receptors. They are characterized by the presence of an amino-terminal effector domain, which is often either a caspase activation and recruitment domain (CARD) or a pyrin domain (PYD), followed by a NACHT domain and carboxy-terminal leucine-rich repeats (LRR) involved in recognition of pathogen-associated molecular patterns (PAMPs) (1). NLR proteins play a variety of roles during the innate immune response including pathogen sensing, transcriptional activation of proinflammatory cytokines through NF-κB, transcriptional activation of type I interferons through IRFs, and formation of inflammasomes leading to activation of inflammatory caspases (1-7). NLRX1 (CLR11.3/NOD26/NOD5/NOD9) is unique among NLR family members in that it contains an amino-terminal mitochondrial targeting sequence resulting in localization to the mitochondria (8,9). In contrast to most NLR proteins, NLRX1 has been shown to act as a negative regulator of innate immune responses through inhibition of MAVS-Rig-I signaling, as well as inhibition of Toll-like receptor (TLR)-mediated NF-κB activation (9-11). In addition, overexpression of NLRX1 enhanced the production of reactive oxygen species (ROS), resulting in prolonged NF-κB and JNK signaling in response to TNF-α (8). Alternate Names Caterpiller protein 11.3; CLR11.3; DLNB26; FLJ21478; MGC131937; MGC21025; NLR family member X1; NLR family, X1; NLRX1; NOD-like receptor X1; NOD26; NOD5; NOD9; Nucleotide-binding oligomerization domain protein 26; Nucleotide-binding oligomerization domain protein 5; Nucleotide-binding oligomerization domain protein 9; nucleotide-binding oligomerization domain, leucine rich repeat containing X1 Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 100 Source/Isotype: Rabbit IgG