CST, 13848T, DNA Polymerase eta (E1I7T) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying POLH. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, DNA Polymerase eta (E1I7T) Rabbit Monoclonal Antibody (CST #13848) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity DNA Polymerase eta (E1I7T) Rabbit Monoclonal Antibody recognizes endogenous levels of total POLH protein. This antibody recognizes a 40 kDa background band of unknown origin. In some cell lines, this antibody recognizes a 60 kDa band of unknown origin. This band does not correspond to the expected size of truncated POLH in GM03617 cells. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro309 of human POLH protein. Background A total of fifteen mammalian DNA polymerase enzymes catalyze the synthesis of nascent DNA during DNA replication and repair (1). DNA polymerase eta (POL η, POLH, Rad30) is one of a specialized type of DNA polymerases that function in DNA repair and translesion synthesis (TLS). POLH can accommodate and read through bulky DNA lesions such as pyrimidine dimers, which allows for continued DNA synthesis past lesions and limited stalling of replication forks (2,3). Damage inducing conditions, such as exposure to UV light or cisplatin, recruit POLH to sites of bulky DNA lesions where the polymerase interacts with PCNA (4,5). Mutations in the human gene can result in a form of xeroderma pigmentosum (XPV), an autosomal recessive disorder characterized by hypersensitivity to light and susceptibility to skin cancer (6). Alternate Names DNA polymerase eta; FLJ16395; FLJ21978; POLH; polymerase (DNA directed), eta; polymerase (DNA) eta; RAD30; RAD30 homolog A; RAD30A; Xeroderma pigmentosum variant type protein; XP-V; XPV Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 80 Source/Isotype: Rabbit IgG