CST, 13868S, LARS Antibody

Polyclonal Antibody for studying LARS. Validated for Western Blotting. Highly specific and rigorously validated in-house, LARS Antibody (CST #13868) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity LARS Antibody recognizes endogenous levels of total LARS protein. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human LARS protein. Antibodies are purified by protein A and peptide affinity chromatography. Background Leucyl-tRNA Synthetase (LARS) is a leucine sensor critical for the activation of mTORC1 (1). mTORC1 kinase complex is an important component in the regulation of cell growth (2,3). Its activity is modulated by energy levels, growth factors, and amino acids (4,5). The four related GTPases, RagA, RagB, RagC, and RagD, have been shown to interact with raptor in mTORC1 (2,3). These interactions are both necessary and sufficient for mTORC1 activation in response to amino acid signals (2,3). LARS functions as a GTPase-activating protein (GAP) and interacts directly with RagD GTPase (1). The role of LARS in leucine sensing is not related to its tRNA charging activity (1). Alternate Names cytoplasmic leucyl-tRNA synthetase; cytosolic leucyl-tRNA synthetase; FLJ10595; FLJ21788; hr025Cl; HSPC192; ILFS1; KIAA1352; LARS; LARS1; leucine translase; leucine tRNA ligase 1, cytoplasmic; Leucine--tRNA ligase; Leucine--tRNA ligase, cytoplasmic; leucine-tRNA ligase; leucyl-tRNA synthetase; leucyl-tRNA synthetase 1; Leucyl-tRNA synthetase, cytoplasmic; LeuRS; LEUS; LFIS; LRS; PIG44; proliferation-inducing gene 44; RNTLS; SYLC Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 134 SOURCE: Rabbit