CST, 13913S, CAMLG (D5L9J) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying CAMLG. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, CAMLG (D5L9J) Rabbit Monoclonal Antibody (CST #13913) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:200 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity CAMLG (D5L9J) Rabbit Monoclonal Antibody recognizes endogenous levels of total CAMLG protein. Species Reactivity: Human, Mouse, Rat, Hamster, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu110 of human CAMLG protein. Background The calcium signal-modulating cyclophilin ligand (CAMLG) is a multi-pass endoplasmic reticulum (ER) membrane protein that plays an important role in calcium-mediated signal transduction. The CAMLG protein was first identified in T lymphocytes as a cyclophilin B-binding protein that regulates calcium influx following T-cell receptor (TCR) activation (1). Research studies indicate that CAMLG overexpression activates the transcription factors NFAT and NF-IL2A and leads to increased IL-2 gene transcription, providing additional evidence that CAMLG plays an important role in TCR signal transduction. CAMLG negatively regulates the non-receptor protein kinase Lck, which is critical for the thymocyte selection process (2). Thymocytes deficient for CAMLG fail to undergo normal development, accumulate reactive oxygen species, and exhibit increased apoptosis in response to cytotoxic stimuli (3). The CAMLG protein also acts as a receptor of TRC40, an ATPase that targets newly synthesized proteins in the secretory pathway to the ER membrane. CAMLG interacts with the protein insertion receptor WRB to form the TRC40 receptor complex that is responsible for insertion of tail-anchored proteins into the ER membrane (4). Alternate Names calcium modulating ligand; Calcium signal-modulating cyclophilin ligand; calcium-modulating cyclophilin ligand; calcium-signal modulating cyclophilin ligand; CAML; CAMLG; cyclophilin B-binding protein; GET2; Guided entry of tail-anchored proteins factor CAMLG; MGC163197 Specification REACTIVITY: H M R Hm Mk SENSITIVITY: Endogenous MW (kDa): 32 Source/Isotype: Rabbit IgG