CST, 13945S, INTS9 Antibody

Polyclonal Antibody for studying INTS9. Validated for Western Blotting,Immunoprecipitation,Chromatin IP. Highly specific and rigorously validated in-house, INTS9 Antibody (CST #13945) is ready to ship. Product Usage Information For optimal ChIP results, use 10 μl of antibody and 10 μg of chromatin (approximately 4 x 10 6 cells) per IP. This antibody has been validated using SimpleChIP ® Enzymatic Chromatin IP Kits. Western Blotting: 1:1000 Immunoprecipitation: 1:100 Chromatin IP: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation, Chromatin IP Specificity / Sensitivity INTS9 Antibody recognizes endogenous levels of total INTS9 protein. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding His601 of human INTS9 protein. Antibodies are purified by protein A and peptide affinity chromatography. Background The integrator complex is an evolutionarily conserved complex that is composed of at least 12 subunits in humans. It is thought to be a multifunctional complex with roles in orchestrating snRNA 3' end processing with transcription termination, DNA double-stranded break repair, hematopoietic development, and cell cycle progression (1-6). The integrator subunits (INTS) 9 and 11 are thought to be the catalytic subunits of the complex and are essential for the function of the complex (6,7). Research studies indicate that the integrator complex is recruited to snRNA genes through its interaction with the carboxy-terminal domain (CTD) of Rpb1, the largest subunit of RNA polymerase II (8). Phosphorylation of the Rpb1 CTD heptapeptide repeat residues Ser2 and Ser7 is required for efficient binding of integrator subunit proteins (9). Alternate Names CPSF2L; FLJ10871; Int9; Integrator complex subunit 9; INTS9; Protein related to CPSF subunits of 74 kDa; RC-74; RC74 Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 75 SOURCE: Rabbit