CST, 13978T, MDR1/ABCB1 (E1Y7S) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying ABCB1. Validated for Western Blotting,Simple Western™,Immunohistochemistry (Paraffin). Available in 2 sizes. Highly specific and rigorously validated in-house, MDR1/ABCB1 (E1Y7S) Rabbit Monoclonal Antibody (CST #13978) is ready to ship. Product Usage Information Western Blotting: 1:1000 Simple Western™: 1:50 - 1:250 Immunohistochemistry (Paraffin): 1:400 - 1:1600 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 70247 . Protocol Available protocols: Western Blotting, Immunohistochemistry (Paraffin) Specificity / Sensitivity MDR1/ABCB1 (E1Y7S) Rabbit Monoclonal Antibody recognizes endogenous levels of total MDR1 protein. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with recombinant protein surrounding Ala650 of human MDR1 protein. Background MDR1/ABCB1 belongs to the Mdr/Tap subfamily of the ATP-binding cassette transporter superfamily (1). Multidrug resistance 1 (MDR1) serves as an efflux pump for xenobiotic compounds with broad substrate specificity. MDR1 substrates include therapeutic agents such as actinomycin D, etoposide, imatinib, and doxorubicin, as well as endogenous molecules including β-amyloids, steroid hormones, lipids, phospholipids, cholesterol, and cytokines (2). Research studies have shown that MDR1 reduces drug accumulation in cancer cells, allowing the development of drug resistance (3-5). On the other hand, MDR1 expressed in the plasma membrane of cells in the blood-brain, blood-cerebral spinal fluid, or blood-placenta barriers restricts the permeability of drugs into these organs from the apical or serosal side (6,7). MDR1 is also expressed in normal tissues with excretory function such as small intestine, liver, and kidney (7). Intracellular MDR1 has been detected in the ER, vesicles, and nuclear envelope, and has been associated with cell trafficking machinery (8). Other reported functions of MDR1 include viral resistance, cytokine trafficking (9,10), and lipid homeostasis in the peripheral and central nervous system (11-13). Alternate Names ABC20; ABCB1; ATP binding cassette subfamily B member 1; ATP-binding cassette sub-family B member 1; ATP-binding cassette, sub-family B (MDR/TAP), member 1; ATP-binding cassette, subfamily B, member 1; ATP-dependent translocase ABCB1; CD243; CLCS; colchicin sensitivity; doxorubicin resistance; GP170; MDR1; MGC163296; Multidrug resistance protein 1; P-glycoprotein 1; P-GP; PGY1; Phospholipid transporter ABCB1 Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 130-180 Source/Isotype: Rabbit IgG