CST, 13995S, Claudin-1 (D3H7C) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying Claudin-1. Validated for Western Blotting,Immunofluorescence (Immunocytochemistry). Available in 2 sizes. Highly specific and rigorously validated in-house, Claudin-1 (D3H7C) Rabbit Monoclonal Antibody (CST #13995) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunofluorescence (Immunocytochemistry): 1:200 - 1:800 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunofluorescence (Immunocytochemistry) Specificity / Sensitivity Claudin-1 (D3H7C) Rabbit Monoclonal Antibody recognizes endogenous levels of total claudin-1 protein. In both claudin-1-positive and claudin-1-negative cell lines, the antibody cross-reacts with a band of unknown origin, which migrates at approximately 120 kDa by SDS-PAGE electrophoresis. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro194 of human claudin-1 protein. Background Tight junctions, or zonula occludens, form a continuous barrier to fluids across the epithelium and endothelium. They function in regulation of paracellular permeability and in the maintenance of cell polarity, blocking the movement of transmembrane proteins between the apical and the basolateral cell surfaces. Tight junctions are composed of claudin and occludin proteins, which join the junctions to the cytoskeleton (1,2). The claudin family is composed of 23 integral membrane proteins, and their expression, which varies among tissue types, may determine both the strength and properties of the epithelial barrier. Alteration in claudin protein expression pattern is associated with several types of cancer (2,3). Claudin-1 is expressed primarily in keratinocytes (4) and normal mammary epithelial cells, but is absent or reduced in breast carcinomas and breast cancer cell lines (5,6). Alternate Names claudin 1; Claudin-1; claudin1; CLD1; CLDN1; ILVASC; SEMP1; Senescence-associated epithelial membrane protein; senescence-associated epithelial membrane protein 1 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 20 Source/Isotype: Rabbit IgG