CST, 14041S, Phospho-Prdx1 (Tyr194) (D1T9C) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying PRDX1 (Tyr194) phosphate. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, Phospho-Prdx1 (Tyr194) (D1T9C) Rabbit Monoclonal Antibody (CST #14041) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity Phospho-Prdx1 (Tyr194) (D1T9C) Rabbit Monoclonal Antibody recognizes endogenous levels of Prdx1 protein only when phosphorylated at Tyr194. This antibody may cross react with other activated receptor tyrosine kinases including EGFR. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr194 of human Prdx1 protein. Background Prdx1 belongs to a family of non-seleno peroxidases that function as H O scavengers. All 6 Prdx isoforms share a conserved N-terminal cysteine (Cys51) that is oxidized by H O to form cysteine-sulfenic acid (Cys51-SOH) and, in turn, reacts with Cys172-SH of another Prdx protein, forming a disulfide dimer and protecting it from degradation (1-3). Abnormally high levels of H O cause Prdx1 to form an oligomeric chaperone that loses its peroxidase activity (4). Prdx family members have been reported to bind to JNK and c-Abl and regulate their kinase activity (5,6). Prdx1 was shown to bind to PTEN and regulate its phosphatase activity in conditions of mild or no cellular stress, hence preventing Akt-driven transformation by protecting PTEN from oxidation-induced inactivation (7). The transient phosphorylation of Prdx1 at Tyr194 leads to inactivation of Prdx1 that in turn promotes localized hydrogen peroxide accumulation (8). Evidence suggests that the Tyr194 phosphorylation of Prdx1 is prominent at the wound edge during repair of cutaneous injury in mice (8,9). Alternate Names epididymis secretory sperm binding protein; MSP23; Natural killer cell-enhancing factor A; natural killer-enhancing factor A; NKEF-A; NKEFA; PAG; PAGA; PAGB; peroxiredoxin 1; Peroxiredoxin-1; PRDX1; proliferation-associated gene A; Proliferation-associated gene protein; PRX1; PRXI; TDPX2; Thioredoxin peroxidase 2; Thioredoxin-dependent peroxide reductase 2; Thioredoxin-dependent peroxiredoxin 1 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 21 Source/Isotype: Rabbit IgG