CST, 14053T, Phospho-Doublecortin (Ser334) (D11B10) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying Doublecortin (Ser334) phosphate mouse. Validated for Western Blotting,Immunofluorescence (Frozen),Immunofluorescence (Immunocytochemistry). Available in 2 sizes. Highly specific and rigorously validated in-house, Phospho-Doublecortin (Ser334) (D11B10) Rabbit Monoclonal Antibody (CST #14053) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunofluorescence (Frozen): 1:200 Immunofluorescence (Immunocytochemistry): 1:1600 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunofluorescence (Frozen), Immunofluorescence (Immunocytochemistry) Specificity / Sensitivity Phospho-Doublecortin (Ser334) (D11B10) Rabbit Monoclonal Antibody recognizes endogenous levels of doublecortin protein only when phosphorylated at Ser334. Amino acid Ser334 of mouse doublecortin corresponds to Ser415 of human doublecortin. Proteins of unknown origin may be detected around 90 kDa. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser334 of mouse doublecortin protein. Background Mutations in doublecortin ( ) cause Lissencephaly (smooth brain), a neuronal migration disorder characterized by epilepsy and mental retardation (1). Doublecortin is a microtubule-associated protein that stabilizes and bundles microtubules. A conserved doublecortin domain mediates the interaction with microtubules, and interestingly most missense mutations cluster in this domain (2). Kinases JNK, CDK5, and PKA phosphorylate doublecortin. JNK phosphorylates Thr321, Thr331, and Ser334 while PKA phosphorylates Ser47 and CDK5 phosphorylates Ser297 (3-5). Phosphorylation of Ser297 lowers the affinity of doublecortin to microtubules. Furthermore, mutations of Ser297 result in migration defects (5). Doublecortin phosphorylated at Ser334 is enriched in growth cones and affects neurite outgrowth and neuronal migration (3). Alternate Names Dbct; Dcn; Dcx; doublecortex; doublecortin; Doublin; Lis-X; Lissencephalin-X; lissencephaly, X-linked (doublecortin); Neuronal migration protein doublecortin; OTTMUSP00000020397 Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 45 Source/Isotype: Rabbit IgG