CST, 14071S, Cleaved Caspase-8 (Asp387) (D5B2) Rabbit Monoclonal Antibody (PE Conjugate)

Monoclonal Antibody for studying Casp8 (Asp387) cleaved mouse. Validated for Flow Cytometry (Fixed/Permeabilized). Highly specific and rigorously validated in-house, Cleaved Caspase-8 (Asp387) (D5B2) Rabbit Monoclonal Antibody (PE Conjugate) (CST #14071) is ready to ship. Product Usage Information Flow Cytometry (Fixed/Permeabilized): 1:50 Storage Supplied in PBS (pH 7.2), less than 0.1% sodium azide and 2 mg/ml BSA. Store at 4°C. Do not aliquot the antibodies. Protect from light. Do not freeze. Protocol Available protocols: Flow Cytometry (Fixed/Permeabilized) Specificity / Sensitivity Cleaved Caspase-8 (Asp387) (D5B2) Rabbit Monoclonal Antibody (PE Conjugate) recognizes endogenous levels of mouse caspase-8 only when cleaved at Asp387. In addition to the p18 subunit, the unconjugated antibody also detects cleavage products containing the pro-domain with the p18 subunit (p43) by western blot analysis. Species Reactivity: Mouse Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asp387 of mouse caspase-8 protein. Background Apoptosis induced through the CD95 receptor (Fas/APO-1) and tumor necrosis factor receptor 1 (TNFR1) activates caspase-8 and leads to the release of the caspase-8 active fragments, p18 and p10 (1-3). Activated caspase-8 cleaves and activates downstream effector caspases such as caspase-1, -3, -6, and -7. Caspase-3 ultimately elicits the morphological hallmarks of apoptosis, including DNA fragmentation and cell shrinkage. Alternate Names Casp; CASP-8; Casp8; caspase 8; Caspase-8; Caspase-8 subunit p10; Caspase-8 subunit p18; caspase8; Fas-linked ICE-like protease; FLI; FLICE; MAC; MACH; Mch5 Specification REACTIVITY: M SENSITIVITY: Endogenous Source/Isotype: Rabbit IgG