CST, 14087S, CdGAP (D6J9G) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying cdGAP. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, CdGAP (D6J9G) Rabbit Monoclonal Antibody (CST #14087) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity CdGAP (D6J9G) Rabbit Monoclonal Antibody recognizes endogenous levels of total CdGAP protein. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ser520 of human CdGAP protein. Background The Rho family of small GTPases, including Rho, Rac, and Cdc42, act as molecular switches that regulate processes such as cell migration, adhesion, proliferation, and differentiation. They are activated by guanine nucleotide exchange factors (GEFs), which catalyze the exchange of bound GDP for GTP, and inhibited by GTPase activating proteins (GAPs), which catalyze the hydrolysis of GTP to GDP (1). The serine- and proline-rich GAP protein, Cdc42 GAP (CdGAP), has been shown to be a negative regulator of both Cdc42 and Rac1, but not RhoA (2,3). This protein contains three domains: an amino-terminal GAP domain, a central domain, and a carboxy-terminal proline-rich domain containing five Src homology 3 (SH3)-binding sites. It is suggested that threonine and serine phosphorylation within the proline-rich domain likely alters protein-protein interactions and determines the localization of CdGAP (4). Phosphorylation of CdGAP on threonine 776 by both ERK-1 and GSK-3 has been shown to negatively regulate protein activity, possibly by inducing a conformational change within the protein disrupting its ability to bind SH3 domains (4,5). Upregulation of CdGAP has been shown to increase cell proliferation and it has been suggested that this protein may play a role in TGF-β-induced cell growth, motility, and invasion in some breast cancer cells (6). Alternate Names AOS1; ARHGAP31; Cdc42 GTPase-activating protein; CDGAP; KIAA1204; MGC138368; MGC138370; RHG31; Rho GTPase activating protein 31; Rho GTPase-activating protein 31 Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 250 Source/Isotype: Rabbit IgG