CST, 14217T, CYP11A1 (D8F4F) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying CYP11A1. Validated for Western Blotting,Immunoprecipitation,Immunofluorescence (Immunocytochemistry). Available in 2 sizes. Highly specific and rigorously validated in-house, CYP11A1 (D8F4F) Rabbit Monoclonal Antibody (CST #14217) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Immunofluorescence (Immunocytochemistry): 1:400 - 1:800 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation, Immunofluorescence (Immunocytochemistry) Specificity / Sensitivity CYP11A1 (D8F4F) Rabbit Monoclonal Antibody recognizes endogenous levels of total CYP11A1 protein. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human CYP11A1 protein. Background In steroidogenic tissues, such as the adrenal cortex, testis, ovary, and placenta, all steroids are synthesized from the common precursor cholesterol. Two families of steroidogenic enzymes, cytochrome P450 hydroxylase enzymes (CYP) and hydroxysteroid dehydrogenases (HSD), catalyze the production of most steroids. There are six distinct steroid hydroxylases, which are cytochrome P450 enzymes encoded by the steroidogenic gene family (1). The cytochrome P450scc (cholesterol side-chain cleavage enzyme) encoded by catalyzes the first and rate-limiting step in steroidogenesis, conversion of cholesterol into pregnenolone (2). CYP11A1, located in the inner membrane of mitochondria, cooperates with two coenzymes, ferredoxin and ferredoxin reductase, to carry out three successive oxidation-reduction reactions of cholesterol (3-5). In the adrenal cortex, testis, and ovary, CYP11A1 expression is regulated by the cAMP-PKA pathway (6), and the transcription factor SF1/NR5A1 has been shown to play a central role in mediating the cAMP signal on the promoter within steroidogeneic cells of the adrenal cortex and gonads (7). Defects in CYP11A1 are the cause of adrenal insufficiency congenital with 46, XY sex reversal (AICSR), which is a rare disorder that can present as acute adrenal insufficiency in infancy or childhood (8,9). Alternate Names cholesterol 20-22 desmolase; Cholesterol desmolase; cholesterol monooxygenase (side-chain cleaving); Cholesterol side-chain cleavage enzyme, mitochondrial; CP11A; CYP11A; CYP11A1; CYPXIA1; Cytochrome P450 11A1; cytochrome P450 family 11 subfamily A member 1; cytochrome P450 family 11 subfamily A polypeptide 1; cytochrome P450, family 11, subfamily A, polypeptide 1; cytochrome P450, subfamily XIA (cholesterol side chain cleavage); Cytochrome P450(scc); cytochrome P450C11A1; P450SCC; steroid 20-22-lyase Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 50 Source/Isotype: Rabbit IgG