CST, 14219S, Phospho-Tyrosine (P-Tyr-1000) MultiMab® Rabbit Monoclonal Antibody mix (Biotinylated)

Monoclonal Antibody for studying . Validated for Western Blotting. Highly specific and rigorously validated in-house, Phospho-Tyrosine (P-Tyr-1000) MultiMab^® Rabbit Monoclonal Antibody mix (Biotinylated) (CST #14219) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 140 mM NaCl, 3 mM KCl, 10 mM sodium phosphate (pH 7.4) dibasic, 2 mM potassium phosphate monobasic, 2 mg/mL BSA, and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity Phospho-Tyrosine (P-Tyr-1000) MultiMab Species Reactivity: All Species Expected Source / Purification MultiMab® rabbit monoclonal mix antibodies are prepared by combining individual rabbit monoclonal clones in optimized ratios for the approved applications. Each antibody in the mix is carefully selected based on motif recognition and performance in multiple assays. Each mix is engineered to yield the broadest possible coverage of the modification being studied while ensuring a high degree of specificity for the modification or motif. Background Tyrosine phosphorylation plays a key role in cellular signaling (1). Research studies have shown that in cancer, unregulated tyrosine kinase activity can drive malignancy and tumor formation by generating inappropriate proliferation and survival signals (2). Antibodies specific for phospho-tyrosine (3,4) have been invaluable reagents in these studies. The phospho-tyrosine monoclonal antibodies developed by Cell Signaling Technology are exceptionally sensitive tools for studying tyrosine phosphorylation and monitoring tyrosine kinase activity in high throughput drug discovery. Specification REACTIVITY: All SENSITIVITY: Endogenous Source/Isotype: Rabbit IgG