CST, 14243S, FANCB (D9W6S) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying FancB. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, FANCB (D9W6S) Rabbit Monoclonal Antibody (CST #14243) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity FANCB (D9W6S) Rabbit Monoclonal Antibody recognizes endogenous levels of total FANCB protein. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro280 of human FANCB protein. Background Fanconi anemia (FA) is an autosomal recessive genetic disorder resulting in symptoms that include chromosomal breakage, bone marrow failure, hypersensitivity to DNA cross-linking agents (such as mitomycin C), and a predisposition to cancer (1). FANCB is an X-linked member of the FA nuclear complex (FANCA, FANCB, FANCC, FANCE, FANCF, FANCG, FANCM). In response to DNA damage, the FA nuclear complex induces mono-ubiquitination of FANCD2 and FANCI (2). FANCJ/BRIP1, FANCD1/BRCA2, and FANCN/PALB2 are then recruited to sites of DNA damage along with other DNA repair proteins. FA signaling is important in maintenance of chromosome stability and control of mitosis (3). Studies of FANCB knockout embryonic stem cells suggest a role for FANCB in the formation of Rad51 and FANCD2 foci at chromosomal sites of DNA damage (4). Alternate Names FA complementation group B; FA2; FAAP90; FAAP95; FAB; FACB; FANCB; Fanconi anemia complementation group B; Fanconi anemia group B protein; Fanconi anemia-associated polypeptide of 95 kDa; Fanconi anemia, complementation group B; Protein FACB; type 2 Fanconi pancytopenia Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 90 Source/Isotype: Rabbit IgG