CST, 14298S, T-Bet/TBX21 (D6N8B) Rabbit Monoclonal Antibody (Alexa Fluor® 488 Conjugate)

Monoclonal Antibody for studying TBX21. Validated for Immunofluorescence (Immunocytochemistry),Flow Cytometry (Fixed/Permeabilized). Highly specific and rigorously validated in-house, T-Bet/TBX21 (D6N8B) Rabbit Monoclonal Antibody (Alexa Fluor^® 488 Conjugate) (CST #14298) is ready to ship. Product Usage Information Immunofluorescence (Immunocytochemistry): 1:50 - 1:100 Flow Cytometry (Fixed/Permeabilized): 1:50 Storage Supplied in PBS (pH 7.2), less than 0.1% sodium azide and 2 mg/ml BSA. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze. Protocol Available protocols: Immunofluorescence (Immunocytochemistry), Flow Cytometry (Fixed/Permeabilized) Specificity / Sensitivity T-Bet/TBX21 (D6N8B) Rabbit Monoclonal Antibody (Alexa Fluor Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly465 of human T-Bet/TBX21 protein. Background The gene family consists of transcription factors characterized by a related DNA-binding domain (T-box) of approximately 200 amino acids (1,2). The genes exhibit diverse temporal and spatial patterns in the developing embryo. Studies have demonstrated members of this family play crucial roles during embryogenesis in a wide range of organisms by regulating cell fate decisions to establish the early body plan and to regulate later processes underlying organogenesis (3-5). Mutations in genes are associated with many developmental defects (6). Recent studies also indicate potential roles in cancer by members of the T-box family (7-9). Alternate Names T-bet; T-box 21; T-box expressed in T cells; T-box protein 21; T-box transcription factor 21; T-box transcription factor TBX21; T-cell-specific T-box transcription factor T-bet; T-PET; TBET; TBLYM; TBX21; Transcription factor TBLYM Specification REACTIVITY: H SENSITIVITY: Endogenous Source/Isotype: Rabbit IgG