CST, 14340T, Caspase-11 (17D9) Rat Monoclonal Antibody

Monoclonal Antibody for studying CASP4 mouse. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, Caspase-11 (17D9) Rat Monoclonal Antibody (CST #14340) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:100 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity Caspase-11 (17D9) Rat Monoclonal Antibody recognizes endogenous levels of total caspase-11 protein. Species Reactivity: Mouse Source / Purification Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the p30 subunit terminus of mouse caspase-11 protein. Background Caspases are a family of aspartate-specific cysteine-dependent proteases that play a critical role in apoptosis as well as inflammatory responses. Pro-inflammatory caspases include caspase-1 and mouse caspase-11 (1). Caspase-11 has about 60% and 55% identity to human caspases-4 and -5, respectively. Caspase-1 cleaves inflammatory cytokines such as pro-IL-1β and IL-18 into their mature forms (2). It is activated by proteolytic cleavage producing a tetramer of its two active subunits, p20 and p10. Canonical activation of caspase-1 occurs through several complex molecular platforms designated "inflammasomes" that include Pycard/Asc, nucleotide-binding oligomerization receptors (NLRs), and AIM2 (3, 4). Non-canonical activation of caspase-1 is triggered by caspase-11, which is transcriptionally induced by toll-like receptor ligands including LPS. Activation of this pathway induces inflammatory cytokines as well as pyroptosis, a form of programmed cell death (5-9). Alternate Names Cas; CASP-11; CASP-4; Casp11; Casp4; Caspa; caspase 11, apoptosis-related cysteine peptidase; caspase 11, apoptosis-related cysteine protease; caspase 4, apoptosis-related cysteine peptidase; caspase 4, apoptosis-related cysteine protease; Caspase-11; caspase-11 short form splicing; Caspase-4; Caspase-4 subunit p10; Caspase-4 subunit p20; Caspase11; Caspase4; Caspl; ich-; ich-3; Ich3; Protease ICH-3 Specification REACTIVITY: M SENSITIVITY: Endogenous MW (kDa): 38, 43 Source/Isotype: Rat IgG2a