CST, 14371T, Phospho-CDK Substrate [pTPXK] (D9V5N) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying . Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, Phospho-CDK Substrate [pTPXK] (D9V5N) Rabbit Monoclonal Antibody (CST #14371) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:100 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity Phospho-CDK Substrate [pTPXK] (D9V5N) Rabbit Monoclonal Antibody recognizes endogenous levels of proteins that are phosphorylated at threonine within the context of a TPXK motif. This antibody does not cross-react with non-phosphorylated proteins or phospho-threonine in a different context. Species Reactivity: All Species Expected Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide library containing the phospho-TPXK motif. Background Cyclin-dependent kinases (CDKs) are a family of Ser/Thr kinases that regulate cell-cycle transition through their association and subsequent phosphorylation of substrate proteins in a proline-directed fashion. CDKs phosphorylate a serine or threonine residue within the (S/T)PX(R/K) consensus sequence (1-5). Cell Signaling Technology has developed antibodies to phosphorylated proline-rich motifs for broad-based discovery applications. The TPXK motif is targeted for phosphorylation by CDKs 1, 2, 4, 5, and 6 (1-5). Specification REACTIVITY: All SENSITIVITY: Endogenous Source/Isotype: Rabbit IgG