CST, 14390S, Phospho-Ser-Pro-Pro Motif [pSPP] Rabbit Monoclonal Antibody

Monoclonal Antibody for studying . Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, Phospho-Ser-Pro-Pro Motif [pSPP] Rabbit Monoclonal Antibody (CST #14390) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity Phospho-Ser-Pro-Pro Motif [pSPP] Rabbit Monoclonal Antibody recognizes endogenous levels of proteins only when phosphorylated at serine within the context of the SPP motif. This antibody does not cross-react with proteins phosphorylated at threonine, or with serine residues phosphorylated in other contexts. Species Reactivity: All Species Expected Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide library containing the phospho-Ser-Pro-Pro motif. Background The MAPK and CDK families of serine/threonine protein kinases play important roles in cell signaling and cell cycle control. These kinases phosphorylate threonine or serine followed by a proline residue (1-6). To facilitate the study and discovery of new MAPK and CDK substrates, Cell Signaling Technology has developed antibodies that bind to phospho-threonine or phospho-serine followed by proline. The S/TPP motif matches the consensus sequence for targeted phosphorylation by Cdc2 (7,8), ERK1, ERK2 (9), SAPK/JNK, CDK5, and GSK3 (10). Specification REACTIVITY: All SENSITIVITY: Endogenous Source/Isotype: Rabbit IgG