CST, 14479T, CYR61 (D4H5D) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying CYR61. Validated for Western Blotting,Immunofluorescence (Immunocytochemistry),Flow Cytometry (Fixed/Permeabilized). Available in 2 sizes. Highly specific and rigorously validated in-house, CYR61 (D4H5D) Rabbit Monoclonal Antibody (CST #14479) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunofluorescence (Immunocytochemistry): 1:50 - 1:100 Flow Cytometry (Fixed/Permeabilized): 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 56358 . Protocol Available protocols: Western Blotting, Immunofluorescence (Immunocytochemistry), Flow Cytometry (Fixed/Permeabilized) Specificity / Sensitivity CYR61 (D4H5D) Rabbit Monoclonal Antibody recognizes endogenous levels of total CYR61 protein. This antibody does not cross-react with other CCN-family proteins. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro171 of human CYR61 protein. Background Cysteine-rich protein 61 (CYR61, CCN1) is a secreted, matrix-associated protein belonging to the CCN family, a protein group characterized primarily by its high cysteine content (1). CYR61 regulates diverse cellular events including cell proliferation, differentiation, angiogenesis, and extracellular matrix formation. Research studies have implicated CYR61 in the development or progression of various cancers, including breast, prostate, lung, and hepatocellular carcinoma (1-4). Notably, its role in promoting cancer progression appears to be context-dependent. For example, investigators have shown that overexpression of CYR61 was positively associated with invasiveness of breast cancer cell lines (2), whereas in primary prostate tumors, expression levels were inversely correlated with tumor aggressiveness (3). In additional research studies of hepatocellular carcinoma, where CYR61 expression was positively associated with cancer progression, CYR61 was shown to be transcriptionally regulated by the Wnt/β-catenin signaling pathway (1). Alternate Names CCN family member 1; CCN1; cellular communication network factor 1; CYR61; cysteine rich angiogenic inducer 61; Cysteine-rich angiogenic inducer 61; cysteine-rich heparin-binding protein 61; cysteine-rich, angiogenic inducer, 61; cysteine-rich, anigogenic inducer, 61; GIG1; IBP-10; IGF-binding protein 10; IGFBP-10; IGFBP10; Insulin-like growth factor-binding protein 10; Protein CYR61; Protein GIG1 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 41 Source/Isotype: Rabbit IgG