CST, 14522S, ARFGAP1 (D9A4V) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying ARFGAP1. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, ARFGAP1 (D9A4V) Rabbit Monoclonal Antibody (CST #14522) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity ARFGAP1 (D9A4V) Rabbit Monoclonal Antibody recognizes endogenous levels of total ARFGAP1 protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Thr338 of human ARFGAP1 protein. Background ADP-ribosylation factor GTPase activating protein 1 (ARFGAP1) is a Golgi-localized protein that regulates vesicle formation and membrane trafficking (1). ARFGAP1 initiates cargo selection and COP1 vesicle formation by stimulating GTP hydrolysis of ADP-ribosylation factor ARF1 (2). This GTPase activating protein initiates vesicle transport by coupling vesicle formation with cargo sorting (3). ARFGAP1 plays an active role in the Golgi-to-ER retrograde, intra-Golgi, and trans-Golgi trafficking networks (1). Research studies indicate that ARFGAP1 can act as a GTPase activating protein for LRRK2, a large multifunction protein whose genetic mutations are associated with Parkinson's disease (4). ARFGAP1 regulates GTPase activity and promotes the kinase activity of LRRK2, which suggests some potential as a promising target for study of LRRK2 mediated neurodegeneration (4). Alternate Names ADP ribosylation factor GTPase activating protein 1; ADP-ribosylation factor 1 GTPase activating protein; ADP-ribosylation factor 1 GTPase-activating protein; ADP-ribosylation factor GTPase activating protein 1; ADP-ribosylation factor GTPase-activating protein 1; ARF GAP 1; ARF GAP1; ARF1 GAP; ARF1-directed GTPase-activating protein; ARF1GAP; ARFG1; ARFGAP1; GAP protein; HRIHFB2281; MGC39924 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 45 Source/Isotype: Rabbit IgG