CST, 14528T, Aromatase (D5Q2Y) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying Aromatase. Validated for Western Blotting,Immunoprecipitation,Immunohistochemistry (Paraffin). Available in 2 sizes. Highly specific and rigorously validated in-house, Aromatase (D5Q2Y) Rabbit Monoclonal Antibody (CST #14528) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Immunohistochemistry (Paraffin): 1:4200 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 64970 . Protocol Available protocols: Western Blotting, Immunoprecipitation, Immunohistochemistry (Paraffin) Specificity / Sensitivity Aromatase (D5Q2Y) Rabbit Monoclonal Antibody recognizes endogenous levels of total aromatase protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human aromatase protein. Background Aromatase is a member of the cytochrome P450 superfamily of enzymes, which are monooxygenases that catalyze reactions involved in drug metabolism and cholesterol and steroid synthesis (1,2). Aromatase is responsible for the conversion of testosterone into 17-β estradiol (2). Aromatase is mainly expressed in the brain (3), ovaries (4), and placenta (5). Aromatase plays an important role in development of the central nervous system during ontogenesis (6,7), gonadal development, and sex differentiation (8,9). Research studies have suggested that inhibition of aromatase may be an effective therapeutic strategy for postmenopausal breast cancers that are estrogen receptor positive (6,10). Mutations in the corresponding aromatase gene are associated with cases of aromatase excess syndrome (AEXS) and aromatase deficiency (AROD) disorders (11-14). Alternate Names ARO; ARO1; Aromatase; CP19A; CPV1; CYAR; CYP19; CYP19A1; CYPXIX; Cytochrome P-450AROM; Cytochrome P450 19A1; cytochrome P450 family 19 subfamily A member 1; cytochrome P450, family 19, subfamily A, polypeptide 1; cytochrome P450, subfamily XIX (aromatization of androgens); Estrogen synthase; estrogen synthetase; flavoprotein-linked monooxygenase; MGC104309; microsomal monooxygenase; P-450AROM Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 50 Source/Isotype: Rabbit IgG