CST, 14531S, Phospho-DDR1 (Tyr513) (E1N8F) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying DDR1 (Tyr513) Phosphate. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, Phospho-DDR1 (Tyr513) (E1N8F) Rabbit Monoclonal Antibody (CST #14531) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity Phospho-DDR1 (Tyr513) (E1N8F) Rabbit Monoclonal Antibody recognizes endogenous levels of DDR1 protein only when phosphorylated at Tyr513. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr513 of human DDR1 protein. Background The discoidin domain receptors (DDRs) are receptor tyrosine kinases with a discoidin homology repeat in their extracellular domains, activated by binding to extracellular matrix collagens. So far, two mammalian DDRs have been identified: DDR1 and DDR2 (1). They are widely expressed in human tissues and may have roles in smooth muscle cell-mediated collagen remodeling (2). Research studies have implicated aberrant expression and signaling of DDRs in human diseases related to increased matrix degradation and remodeling, such as cardiovascular disease, liver fibrosis, and tumor invasion (1). Phosphorylation of DDR1 on Tyr513 was identified at Cell Signaling Technology (CST) using PhosphoScan , a CST™ LC-MS/MS platform for phosphorylation site discovery (3). Additional research looking at DDR1 activation state has identified the same phosphorylation site in DDR1 (4). Alternate Names CAK; CD167; CD167 antigen-like family member A; CD167a; Cell adhesion kinase; DDR; DDR1; discoidin domain receptor family, member 1; discoidin domain receptor tyrosine kinase 1; Discoidin receptor tyrosine kinase; EDDR1; Epithelial discoidin domain receptor 1; Epithelial discoidin domain-containing receptor 1; HGK2; mammarian carcinoma kinase 10; Mammary carcinoma kinase 10; MCK-10; MCK10; NEP; neuroepithelial tyrosine kinase; neurotrophic tyrosine kinase, receptor, type 4; NTRK4; Protein-tyrosine kinase 3A; Protein-tyrosine kinase RTK-6; PTK3; PTK3A; PTK3A protein tyrosine kinase 3A; RTK6; TRK E; TRKE; Tyrosine kinase DDR; Tyrosine-protein kinase CAK Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 125 Source/Isotype: Rabbit IgG