CST, 14534T, IGF-I Receptor beta (D4O6W) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying IGF1R beta. Validated for Western Blotting,IHC Leica Bond,Immunohistochemistry (Paraffin). Available in 2 sizes. Highly specific and rigorously validated in-house, IGF-I Receptor beta (D4O6W) Rabbit Monoclonal Antibody (CST #14534) is ready to ship. Product Usage Information Western Blotting: 1:1000 IHC Leica Bond: 1:50 - 1:200 Immunohistochemistry (Paraffin): 1:400 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 10057 . Protocol Available protocols: Western Blotting, IHC Leica Bond, Immunohistochemistry (Paraffin) Specificity / Sensitivity IGF-I Receptor beta (D4O6W) Rabbit Monoclonal Antibody recognizes endogenous levels of total IGF-I receptor β protein, which includes both unprocessed and processed forms of the protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with recombinant protein specific to human IGF-I receptor β protein. Background Type I insulin-like growth factor receptor (IGF-IR) is a transmembrane receptor tyrosine kinase that is widely expressed in many cell lines and cell types within fetal and postnatal tissues (1-3). Receptor autophosphorylation follows binding of the IGF-I and IGF-II ligands. Three tyrosine residues within the kinase domain (Tyr1131, Tyr1135, and Tyr1136) are the earliest major autophosphorylation sites (4). Phosphorylation of these three tyrosine residues is necessary for kinase activation (5,6). Insulin receptors (IRs) share significant structural and functional similarity with IGF-I receptors, including the presence of an equivalent tyrosine cluster (Tyr1146/1150/1151) within the kinase domain activation loop. Tyrosine autophosphorylation of IRs is one of the earliest cellular responses to insulin stimulation (7). Autophosphorylation begins with phosphorylation at Tyr1146 and either Tyr1150 or Tyr1151, while full kinase activation requires triple tyrosine phosphorylation (8). Alternate Names CD221; IGF-I receptor; IGF1R; IGFIR; IGFR; insulin like growth factor 1 receptor; Insulin-like growth factor 1 receptor; Insulin-like growth factor 1 receptor alpha chain; Insulin-like growth factor 1 receptor beta chain; Insulin-like growth factor I receptor; JTK13; MGC142170; MGC142172; MGC18216; soluble IGF1R variant 1; soluble IGF1R variant 2 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 95, 200 Source/Isotype: Rabbit IgG