CST, 14550S, MATE1/SLC47A1 (D4C6Z) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying MATE1. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, MATE1/SLC47A1 (D4C6Z) Rabbit Monoclonal Antibody (CST #14550) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity MATE1/SLC47A1 (D4C6Z) Rabbit Monoclonal Antibody recognizes endogenous levels of total MATE1 protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu505 of human MATE1 protein. Background The multidrug and toxin extrusion protein 1 (MATE1, SLC47A1) is a proton-coupled, organic cation antiporter located at the apical membrane of proximal kidney epithelial cells and the canalicular membrane of hepatocytes (1). MATE1 mediates the secretion of organic cations including drugs, toxins, and endogenous metabolites, into bile and urine (2,3). Substrates of MATE1 include multiple therapeutic agents, including metformin, cisplatin, acyclovir, and cephalexin (4,5). Polymorphisms in the corresponding gene may affect the rate of renal clearance of certain cationic drugs, limiting the therapeutic benefits of these agents (6). Specifically, research studies demonstrate that allelic variation correlates with differences in renal clearance rates of metformin (7), which may have an effect on the therapeutic impact of this drug in individuals diagnosed with type 2 diabetes (8). Alternate Names FLJ10847; hMATE-1; MATE-1; MATE1; MGC64822; multidrug and toxin extrusion 1; Multidrug and toxin extrusion protein 1; S47A1; SLC47A1; solute carrier family 47 (multidrug and toxin extrusion), member 1; Solute carrier family 47 member 1; solute carrier family 47, member 1 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 48-52 Source/Isotype: Rabbit IgG