CST, 14568S, RPL5 Antibody

Polyclonal Antibody for studying RPL5. Validated for Western Blotting. Highly specific and rigorously validated in-house, RPL5 Antibody (CST #14568) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity RPL5 Antibody recognizes endogenous levels of total RPL5 protein. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gln122 of human RPL5 protein. Antibodies are purified by protein A and peptide affinity chromatography. Background Ribosomal protein L5 (RPL5) is one of several proteins that comprise the 60S ribosomal subunit. RPL5 binds 5S rRNA and the nucleolar RPL11 protein to form the 5S ribonucleoprotein particle (RNP) that is incorporated into the large 60S ribosomal subunit (1). An RP-MDM2-p53 protein complex that contains ribosomal proteins RPL5, RPL11, and RPL23 acts as a nucleolar stress sensor that binds and inhibits MDM2 ubiquitin ligase activity and enhances p53-mediated transcriptional activity (2,3). RPL5 cooperates with RPL11 to influence ribosome biogenesis through regulating expression of the transcription factor c-Myc, which acts as the master regulator of ribosome biogenesis (4). Mutations in the corresponding gene are associated with Diamond-Blackfan anemia, which is a form of red blood cell aplasia, and some cases of pediatric T-cell acute lymphoblastic leukemia (5,6). Alternate Names 60S ribosomal protein L5; DBA6; L5; Large ribosomal subunit protein uL18; MGC117339; MSTP030; PPP1R135; protein phosphatase 1, regulatory subunit 135; ribosomal protein L5; RL5; RPL5; uL18 Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 34 SOURCE: Rabbit