CST, 14602S, GMPS Antibody

Polyclonal Antibody for studying GMPS. Validated for Western Blotting. Highly specific and rigorously validated in-house, GMPS Antibody (CST #14602) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity GMPS Antibody recognizes endogenous levels of total GMPS protein. Species Reactivity: Human, Mouse, Rat Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human GMPS protein. Antibodies are purified by protein A and peptide affinity chromatography. Background Guanine monophosphate synthase (GMPS) catalyzes the conversion of xanthosine 5'-monophosphate (XMP) into GMP during the final step of guanine nucleotide synthesis. In addition to comprising the building blocks for DNA and RNA, guanine nucleotides also provide GTP for many signaling pathways that are important for multiple cellular processes (1). Chromosomal translocations involving MLL and GMPS is reported in some patients diagnosed with acute myeloid leukemia (2). Research studies indicate that genotoxic stress or nucleotide deprivation prompts translocation of GMPS to the nucleus where it complexes with USP7 and p53 to promote p53 stabilization (3). Additional studies show that the nuclear GMPS-USP7 protein complex deubiquitinates histone H2B in (4). Alternate Names GATD7; Glutamine amidotransferase; GMP synthase; GMP synthase [glutamine-hydrolyzing]; GMP synthetase; GMPS; GUAA; guanine monophosphate synthase; guanine monophosphate synthetase; guanine monphosphate synthetase; guanosine 5'-monophosphate synthase; MLL/GMPS fusion partner; MLL/GMPS fusion protein; testicular tissue protein Li 82 Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 76 SOURCE: Rabbit