CST, 14607S, XPA (D9U5U) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying XPA. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, XPA (D9U5U) Rabbit Monoclonal Antibody (CST #14607) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity XPA (D9U5U) Rabbit Monoclonal Antibody recognizes endogenous levels of total XPA protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Arg158 of human XPA protein. Background Nucleotide excision repair (NER) is a process by which cells identify and repair DNA lesions that result from chemical and radiation exposure (1). The DNA binding protein XPA is an essential part of a pre-incision complex that forms at sites of damage, and is necessary for the initiation of nucleotide excision repair (2). XPA is one of eight NER proteins (XPA-G, XPV) encoded by genes that are defective in cases of xeroderma pigmentosum, a disorder characterized by sensitivity to sunlight, predisposition to exposed tissue cancers, and neurological defects in some patients (3). Activation of XPA follows phosphorylation at Ser196 and results in increased NER activity. Phosphorylation of XPA at Ser196 is induced by UV exposure in an ATR-dependant fashion (4) and promotes nuclear accumulation of XPA (5). Research studies suggest that XPA may be a direct substrate of the serine/threonine kinase ATR (4) and that NER activity may be negatively regulated through dephosphorylation of Ser196 by the phosphatase WIP1 (6). Alternate Names DNA repair protein complementing XP-A cells; excision repair-controlling; Xeroderma pigmentosum group A-complementing protein; xeroderma pigmentosum, complementation group A; XP1; XPA; XPA, DNA damage recognition and repair factor; XPAC Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 40 Source/Isotype: Rabbit IgG