CST, 14613S, MKL2/MRTF-B Antibody

Polyclonal Antibody for studying MKL2. Validated for Western Blotting,Immunoprecipitation. Highly specific and rigorously validated in-house, MKL2/MRTF-B Antibody (CST #14613) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity MKL2/MRTF-B Antibody recognizes endogenous levels of total MKL2/MRTF-B protein. Species Reactivity: Human, Mouse Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gln1081 of human MKL2/MRTF-B protein. Antibodies are purified by protein A and peptide affinity chromatography. Background The megakaryoblastic leukemia proteins 1 and 2 (MKL1, MKL2) are myocardin-related transcription factors (MRTF-A, MRTF-B) that serve as actin-regulated transcription coactivators for the serum response factor (SRF). Interaction between G-actin and MKL proteins retains the coactivator within the cytoplasm of resting cells. Activated Rho-A promotes F-actin assembly and a reduction of the G-actin pool in serum-stimulated cells. This results in the accumulation of MKL proteins in the nucleus, where the coactivator associates with the SRF to activate target gene transcription and mediate multiple cellular processes (1-4). A number of other signaling pathways, including the TGFβ, BMP, and PDGF pathways, also make use of MKL-mediated activation of target gene transcription (5-9). Chromosomal translocations involving the genes encoding MKL1 and MKL2 have been identified in several cases of acute megakaryoblastic leukemia and chondroid lipoma (10-12). Alternate Names CTA-276F8.1; DKFZp686J1745; FLJ31823; FLJ45623; KIAA1243; Megakaryoblastic leukemia 2; MKL and myocardin family member 2; MKL/myocardin like 2; MKL/myocardin-like 2; MKL/myocardin-like protein 2; MKL1/myocardin like 2; MKL2; MRTF-B; MRTFB; myocardin related transcription factor B; Myocardin-related transcription factor B; NPD001 Specification REACTIVITY: H M SENSITIVITY: Endogenous MW (kDa): 145 SOURCE: Rabbit