CST, 14627S, NT5E/CD73 (D7F9A) Rabbit Monoclonal Antibody (Alexa Fluor® 488 Conjugate)

Monoclonal Antibody for studying NT5E. Validated for Immunohistochemistry (Paraffin). Highly specific and rigorously validated in-house, NT5E/CD73 (D7F9A) Rabbit Monoclonal Antibody (Alexa Fluor^® 488 Conjugate) (CST #14627) is ready to ship. Product Usage Information Immunohistochemistry (Paraffin): 1:100 - 1:400 Storage Supplied in PBS (pH 7.2), less than 0.1% sodium azide and 2 mg/mL BSA. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze. Protocol Available protocols: Immunohistochemistry (Paraffin) Specificity / Sensitivity NT5E/CD73 (D7F9A) Rabbit Monoclonal Antibody (Alexa Fluor Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the amino terminus of human NT5E/CD73 protein. Background Ecto-5'-nucleotidase (NT5E, also called CD73) is a 70 kDa glycosyl phosphatidylinositol-anchored, membrane-bound glycoprotein that catalyzes the hydrolysis of extracellular nucleoside monophosphates into bioactive nucleosides (1). NT5E catalyzes the terminal step of extracellular adenosine formation from adenosine monophosphate, which drives the regulation of extracellular adenosine levels and the downstream activation of the four G protein-coupled adenosine receptors (2). Binding of hypoxia-inducible factor (HIF-1) to the NT5E gene promoter leads to upregulation of NT5E during hypoxia (3). The biological roles of NT5E include lymphocyte adhesion (4,5), fibrosis (6), and the regulation of nociception (7,8). Alternate Names 5' nucleotidase (CD73); 5'-NT; 5'-nucleotidase; 5'-nucleotidase ecto; 5'-nucleotidase, ecto (CD73); 5NTD; CALJA; CD73; E5NT; Ecto-5'-nucleotidase; eN; eNT; NT; NT5; NT5E; NTE; Purine 5-Prime-Nucleotidase Specification REACTIVITY: H M R SENSITIVITY: Endogenous Source/Isotype: Rabbit IgG