CST, 14629S, CLASP2 (D5K3E) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying CLASP2. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, CLASP2 (D5K3E) Rabbit Monoclonal Antibody (CST #14629) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity CLASP2 (D5K3E) Rabbit Monoclonal Antibody recognizes endogenous levels of total CLASP2 protein. This antibody also cross-reacts with an unidentified protein of 95 kDa. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala289 of human CLASP2 protein. Background Microtubules (MTs) are polarized cellular filaments composed of α/β tubulin heterodimers. The slower growing (minus) microtubule ends are located at MT organizing centers (MTOCs), with the faster growing (plus) ends extending to the cell periphery. The regulation of MT dynamics is an important part of several biological processes, including cell division, migration, adhesion, membrane trafficking, and polarity (1). Human cytoplasmic linker-associate proteins 1 and 2 (CLASP1 and CLASP2) are evolutionarily conserved proteins that localize to the plus ends of interphase microtubules. During mitosis, CLASP 1 and CLASP2 localize to the centrosomes and kinetochores (KT) where they regulate mitotic spindle positioning to ensure proper chromosome alignment (2,3). Research studies indicate that phosphorylation of the carboxy terminus of CLASP2 during mitosis by CDK1 and PLK1 is required for efficient mitotic MT-KT attachment (4). Phosphorylation of CLASP2 at Ser1013 is a critical step that primes CLASP2 for further phosphorylation by PLK1 (4). The additional phosphorylation of CLASP2 at Ser533 and Ser537 by GSK3-3β controls the distribution of CLASP2 on MTs by inhibiting CLASP2 interaction with the Rac1/cdc42 effector protein IQGAP1 (5). Alternate Names CLAP2; CLASP2; CLIP-associating protein 2; CLIP-associating protein CLASP2; cytoplasmic linker associated protein 2; Cytoplasmic linker-associated protein 2; hOrbit2; KIAA0627; multiple asters (Mast)-like homolog 2; Protein Orbit homolog 2 Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 165 Source/Isotype: Rabbit IgG