CST, 14682S, Digoxigenin (D8Q9J) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying . Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, Digoxigenin (D8Q9J) Rabbit Monoclonal Antibody (CST #14682) is ready to ship. Product Usage Information Western Blotting: 1:20000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity Digoxigenin (D8Q9J) Rabbit Monoclonal Antibody recognizes digoxigenin conjugated molecules. Species Reactivity: All Species Expected Source / Purification Monoclonal antibody is produced by immunizing animals with KLH-conjugated digoxigenin. Background Digoxigenin (DIG) is a highly immunogenic steroid derived from "foxglove" plants of the genus (1). This small hapten molecule can be covalently linked to proteins, nucleic acids, compounds, and glycans. Antibodies targeted against the DIG hapten display high binding affinity to DIG-conjugated molecules and can be used in molecular biology assays, including hybridization, western blot, ELISA, and TUNEL assays (2-4). Specification REACTIVITY: All SENSITIVITY: Transfected Only Source/Isotype: Rabbit IgG