CST, 14694S, VCAM-1 Antibody

Polyclonal Antibody for studying VCAM1 mouse. Validated for Western Blotting. Highly specific and rigorously validated in-house, VCAM-1 Antibody (CST #14694) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity VCAM-1 Antibody recognizes endogenous levels of total VCAM-1 protein. This antibody recognizes both long and short isoforms of VCAM-1 protein. Species Reactivity: Mouse, Rat Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gln203 of mouse VCAM-1 protein. Antibodies are purified by protein A and peptide affinity chromatography. Background VCAM-1 (vascular cell adhesion molecule-1) is a transmembrane glycoprotein containing multiple amino-terminal extracellular Ig-like domains, a transmembrane domain, and a short carboxy-terminal cytoplasmic domain (1). Alternative splicing generates two isoforms of VCAM-1 (2). The role of VCAM-1 during infection and inflammatory diseases is well characterized. Expression of VCAM-1 is induced in endothelial cells by inflammatory cytokines including TNF-α and IL-1β (1). VCAM-1 on endothelial cells interacts with the integrin VLA-4 (α4β1) on leukocytes to mediate migration of circulating leukocytes from the blood across the endothelium and into tissues (3). Alternate Names CD106; V-CAM 1; vascular cell adhesion molecule 1; Vascular cell adhesion protein 1; Vcam; VCAM-1; Vcam1 Specification REACTIVITY: M R SENSITIVITY: Endogenous MW (kDa): 35-45, 85-100 SOURCE: Rabbit