CST, 14699S, Ret (E1N9A) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying Ret. Validated for Western Blotting,Immunoprecipitation,Flow Cytometry (Fixed/Permeabilized). Available in 2 sizes. Highly specific and rigorously validated in-house, Ret (E1N9A) Rabbit Monoclonal Antibody (CST #14699) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Flow Cytometry (Fixed/Permeabilized): 1:200 - 1:800 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 56596 . Protocol Available protocols: Western Blotting, Immunoprecipitation, Flow Cytometry (Fixed/Permeabilized) Specificity / Sensitivity Ret (E1N9A) Rabbit Monoclonal Antibody recognizes endogenous levels of total Ret protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro320 of human Ret protein. Background The Ret proto-oncogene (c-Ret) is a receptor tyrosine kinase that functions as a multicomponent receptor complex in conjunction with other membrane-bound, ligand-binding GDNF family receptors (1). Ligands that bind the Ret receptor include the glial cell line-derived neurotrophic factor (GDNF) and its congeners neurturin, persephin, and artemin (2-4). Research studies have shown that alterations in the corresponding gene are associated with diseases including papillary thyroid carcinoma, multiple endocrine neoplasia (type 2A and 2B), familial medullary thyroid carcinoma, and a congenital developmental disorder known as Hirschsprung's disease (1,3). The Tyr905 residue located in the Ret kinase domain plays a crucial role in Ret catalytic and biological activity. Substitution of Phe for Tyr at position 905 dramatically inhibits Ret autophosphorylation activity (5). Alternate Names Cadherin family member 12; cadherin-related family member 16; CDHF12; CDHR16; Extracellular cell-membrane anchored RET cadherin 120 kDa fragment; HSCR1; MEN2A; MEN2B; MTC1; Proto-oncogene c-Ret; Proto-oncogene tyrosine-protein kinase receptor Ret; PTC; rearranged during transfection; RET; ret proto-oncogene; ret proto-oncogene (multiple endocrine neoplasia and medullary thyroid carcinoma 1, Hirschsprung disease); RET receptor tyrosine kinase; RET transforming sequence; RET-ELE1; RET51; Soluble RET kinase fragment Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 150, 175 Source/Isotype: Rabbit IgG