CST, 14716S, Phospho-Sin1 (Thr86) (D4U9L) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying SIN1 (Thr86) phosphate. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, Phospho-Sin1 (Thr86) (D4U9L) Rabbit Monoclonal Antibody (CST #14716) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity Phospho-Sin1 (Thr86) (D4U9L) Rabbit Monoclonal Antibody recognizes endogenous levels of Sin1 protein only when phosphorylated at Thr86. Species Reactivity: Human, Mouse Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding phosphorylated Thr86 of human Sin1 protein. Background Cell growth is a fundamental biological process whereby cells accumulate mass and increase in size. The mammalian TOR (mTOR) pathway regulates growth by coordinating energy and nutrient signals with growth factor-derived signals (1). mTOR is a large protein kinase that is a component of two different complexes. The mTOR complex 1 (mTORC1), a target of rapamycin, contains mTOR, GβL, and raptor. mTORC2, insensitive to rapamycin, includes mTOR, GβL, Sin1, and rictor (1). The mTORC2 complex phosphorylates Ser473 of Akt/PKB (2). This phosphorylation is essential for full Akt/PKB activation. Furthermore, rictor siRNA knockdown inhibits Ser473 phosphorylation in 3T3-L1 adipocytes (3). mTORC2 has also been shown to phosphorylate the rapamycin-resistant mutants of S6K1, another effector of mTOR (4). In addition, phosphorylation of Sin1 at Thr86 by Akt/PKB was shown to regulate the activity of mTORC2 in adipocytes upon stimulation by growth factors (5). Alternate Names JC310; MAPK associated protein 1; MAPKAP1; MEKK2-interacting protein 1; MGC2745; MIP1; Mitogen-activated protein kinase 2-associated protein 1; mitogen-activated protein kinase associated protein 1; mSIN1; ras inhibitor MGC2745; SAPK-interacting protein 1; SIN1; SIN1b; SIN1g; stress-activated map kinase interacting protein 1; Stress-activated map kinase-interacting protein 1; stress-activated protein kinase-interacting 1; Target of rapamycin complex 2 subunit MAPKAP1; TORC2 subunit MAPKAP1 Specification REACTIVITY: H M SENSITIVITY: Endogenous MW (kDa): 78, 74 Source/Isotype: Rabbit IgG