CST, 14736T, LC3C (D3O6P) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying LC3C. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, LC3C (D3O6P) Rabbit Monoclonal Antibody (CST #14736) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity LC3C (D3O6P) Rabbit Monoclonal Antibody recognizes endogenous levels of total LC3C protein. This antibody does not cross react with other LC3 isoforms. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human LC3C protein. Background Autophagy is a catabolic process for the autophagosomic-lysosomal degradation of bulk cytoplasmic contents (1,2). Autophagy is generally activated by conditions of nutrient deprivation, but it has also been associated with a number of physiological processes including development, differentiation, neurodegenerative diseases, infection, and cancer (3). Autophagy marker Light Chain 3 (LC3) was originally identified as a subunit of microtubule-associated proteins 1A and 1B (termed MAP1LC3) (4) and subsequently found to contain similarity to the yeast protein Apg8/Aut7/Cvt5 critical for autophagy (5). Three human LC3 isoforms (LC3A, LC3B, and LC3C) undergo posttranslational modifications during autophagy (6-9). Cleavage of LC3 at the carboxy terminus immediately following synthesis yields the cytosolic LC3-I form. During autophagy, LC3-I is converted to LC3-II through lipidation by a ubiquitin-like system involving Atg7 and Atg3 that allows for LC3 to become associated with autophagic vesicles (6-10). The presence of LC3 in autophagosomes and the conversion of LC3 to the lower migrating form, LC3-II, have been used as indicators of autophagy (11). In general, expression of LC3C protein is lower than the other LC3 isoforms, with the highest LC3C expression observed in lung, placenta, and ovary (7). Alternate Names ATG8J; Autophagy-related protein LC3 C; Autophagy-related ubiquitin-like modifier LC3 C; LC3-like protein 2; LC3C; MAP1 light chain 3-like protein 2; MAP1 light chain 3-like protein 3; MAP1A/MAP1B LC3 C; MAP1A/MAP1B light chain 3 C; MAP1LC3C; microtubule associated protein 1 light chain 3 gamma; Microtubule-associated protein 1 light chain 3 gamma; Microtubule-associated proteins 1A/1B light chain 3C; MLP3C Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 14 Source/Isotype: Rabbit IgG