CST, 14752T, Kv7.2 (D9L5S) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying KCNQ2. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, Kv7.2 (D9L5S) Rabbit Monoclonal Antibody (CST #14752) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity Kv7.2 (D9L5S) Rabbit Monoclonal Antibody recognizes endogenous levels of total Kv7.2 protein. This antibody does not cross-react with Kv7.3 protein. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human Kv7.2 protein. Background The voltage gated potassium channel Kv7.2 (KCNQ2) associates with its family member Kv7.3 (KCNQ3) to form an M-channel that is involved in synaptic input response and sub-threshold excitability of neurons (1). This heteromeric channel generates the M-current, a slowly activating and deactivating potassium conductance that determines the neuronal excitability (2,3). Expression of these two M-channel proteins is mainly seen within the central nervous system, with both Kv7.2 and Kv7.3 expressed post-synaptically in the human cortex and hippocampus (4). The calcium-binding protein calmodulin binds two separate sites in Kv7.2 to influence exit of the channel protein from the endoplasmic reticulum and translocation to the plasma membrane (5). Mutations in the corresponding gene cause benign familial neonatal seizures-1 (BFNS1), an autosomal dominant form of epilepsy characterized by seizure clusters closely following birth (6,7). Alternate Names BFNC; EBN; EBN1; ENB1; HNSPC; KCNA11; KCNQ2; KQT-like 2; KV7.2; KVEBN1; neuroblastoma-specific potassium channel protein; Neuroblastoma-specific potassium channel subunit alpha KvLQT2; potassium channel, voltage gated KQT-like subfamily Q, member 2; Potassium voltage-gated channel subfamily KQT member 2; potassium voltage-gated channel subfamily Q member 2; potassium voltage-gated channel, KQT-like subfamily, member 2; Voltage-gated potassium channel subunit Kv7.2 Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 95 Source/Isotype: Rabbit IgG