CST, 14762T, PRMT7 (D1K6R) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying PRMT7. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, PRMT7 (D1K6R) Rabbit Monoclonal Antibody (CST #14762) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity PRMT7 (D1K6R) Rabbit Monoclonal Antibody recognizes endogenous levels of total PRMT7 protein. Species Reactivity: Human, Mouse, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val233 of human PRMT7 protein. Background Protein arginine N-methyltransferase 7 (PRMT7) is a member of the protein arginine N-methyltransferase (PRMT) family of proteins that catalyze the transfer of a methyl group from S-adenosylmethionine (AdoMet) to a guanidine nitrogen of arginine (1). The three types of PRMTs share the ability to mono-methylate arginine residues, but vary in their ability to generate differential methylation states (1-3). Mono-methylated arginine residues are further methylated by type I PRMTs to generate an asymmetric di-methyl arginine or by type II PRMTs to form a symmetric-dimethyl arginine. Type III methyltransferases are only able to mono-methylate arginine residues (1-3). Research studies indicate that PRMT7 is a type III PRMT that displays substrate specificity for an arginine-X-arginine (RXR) motif surrounded by several basic residues (4,5). PRMT7 interacts with a wide array of protein substrates and likely plays a role in many biological processes including pluripotency, neuronal differentiation, genomic instability, snRNP biogenesis, and breast cancer metastasis (6-11). Alternate Names [Myelin basic protein]-arginine N-methyltransferase PRMT7; ANM7; FLJ10640; Histone-arginine N-methyltransferase PRMT7; KIAA1933; myelin basic protein-arginine N-methyltransferase; PRMT7; protein arginine methyltransferase 7; Protein arginine N-methyltransferase 7; SBIDDS Specification REACTIVITY: H M Mk SENSITIVITY: Endogenous MW (kDa): 78 Source/Isotype: Rabbit IgG