CST, 14763S, LARP1 Antibody

Polyclonal Antibody for studying LARP1. Validated for Western Blotting. Highly specific and rigorously validated in-house, LARP1 Antibody (CST #14763) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity LARP1 Antibody recognizes endogenous levels of total LARP1 protein. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro314 of human LARP1 protein. Antibodies are purified by protein A and peptide affinity chromatography. Background La-related protein 1 (LARP1) is a ubiquitously expressed RNA binding protein that promotes both global and specific mRNA translation in cells (1). LARP1 belongs to the La-related protein family and contains two RNA binding domains, a La motif (LAM), and a neighboring RNA recognition motif-like (RRM-L) domain (1). Research studies indicate that LARP1 acts downstream of mTORC1 to facilitate cell proliferation and growth by promoting global mRNA translation and translation of mRNAs containing a 5'Terminal Oligo-Pyrimidine (5'TOP) motif, which code for translational machinery components (2,3). At the molecular level, LARP1 associates with 5'TOP mRNAs and multiple translation machinery components to positively regulate translation (2,4). Additional studies show that LARP1 expression is upregulated in hepatocellular carcinoma (HCC) patients and that high LARP1 expression in HCC negatively correlates with survival rate (5). Alternate Names KIAA0731; La ribonucleoprotein 1, translational regulator; La ribonucleoprotein domain family member 1; La ribonucleoprotein domain family, member 1; La-related protein 1; Lar1; LARP; LARP1; Lhp1; MGC19556 Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 150 SOURCE: Rabbit