CST, 14764T, Vti1a (D8U3M) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying VTI1A. Validated for Western Blotting,Immunoprecipitation,Immunofluorescence (Immunocytochemistry). Available in 2 sizes. Highly specific and rigorously validated in-house, Vti1a (D8U3M) Rabbit Monoclonal Antibody (CST #14764) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Immunofluorescence (Immunocytochemistry): 1:100 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation, Immunofluorescence (Immunocytochemistry) Specificity / Sensitivity Vti1a (D8U3M) Rabbit Monoclonal Antibody recognizes endogenous levels of total Vti1a protein. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Arg127 of human Vti1a protein. Background Vesicle transport through interaction with t-SNAREs homolog 1 (Vti1) has two protein members, Vti1a and Vti1b. Human Vti1 was first identified as a homolog of the yeast v-SNARE Vti1p and was able to functionally rescue the phenotype of Vti1p-deficient yeast (1). The mammalian proteins Vti1a and Vti1b exhibit distinct but overlapping localization. Vti1a and Vti1b are both localized in the trans-Golgi network, with Vti1a also found in the Golgi apparatus and Vti1b in endosomes (2). Vti1 proteins have been implicated in a number of protein-protein interactions with partners such as VAMP4, syntaxin 6, syntaxin 8, syntaxin 16, and synaptobrevin (2-4). Research studies demonstrate that Vti1a residing on synaptic vesicles is involved in maintaining spontaneous neurotransmitter release (5). Alternate Names MMDS3; MVti1; SNARE Vti1a-beta protein; vesicle transport through interaction with t-SNAREs 1A; Vesicle transport through interaction with t-SNAREs homolog 1A; vesicle transport through interaction with t-SNAREs homolog 1A (yeast); Vesicle transport v-SNARE protein Vti1-like 2; Vti1-rp2; VTI1A; VTI1RP2 Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 26 Source/Isotype: Rabbit IgG