CST, 14766S, Noxa (D8L7U) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying NOXA. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, Noxa (D8L7U) Rabbit Monoclonal Antibody (CST #14766) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:100 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity Noxa (D8L7U) Rabbit Monoclonal Antibody recognizes endogenous levels of total Noxa protein. This antibody also cross-reacts with multiple unidentified proteins, most notably at 35, 50, and 80 kDa. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human Noxa protein. Background Phorbol-12-myristate-13-acetate-induced protein 1 (PMAIP1, Noxa) is a small protein that plays a key role in mediating apoptotic signaling. Noxa is a pro-apoptotic Bcl-2 family protein that contains a single Bcl-2 homology (BH3) domain (1). Members of the "BH3-only" family (e.g., Noxa, Bad, Bim, Puma, Bid, Bik, and Hrk) are highly regulated proteins that induce apoptosis through BH3-dependent interaction with anti-apoptotic Bcl-2 family proteins (2). Noxa localizes to mitochondria and binds the anti-apoptotic proteins Mcl-1 and A1/Bfl-1, but does not bind to Bcl-2 or Bcl-xL (3). The Noxa protein competes with Mcl-1 for binding to mitochondrial Bak protein. Noxa was originally identified as a phorbol ester inducible protein that is highly expressed in adult T-cell leukemia cell lines (4). Several different stimuli, including DNA damage, hypoxia, interferon, viral infection, and double-stranded RNA, induce Noxa expression in cells. Higher levels of Noxa protein are typically found in hematopoietic cells (3,5,6). Alternate Names adult T cell leukemia-derived PMA-responsive; APR; Immediate-early-response protein APR; NOXA; Phorbol-12-myristate-13-acetate-induced protein 1; PMA-induced protein 1; PMAIP1; Protein Noxa Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 10 Source/Isotype: Rabbit IgG