CST, 14769T, IFIT1 (D2X9Z) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying IFIT1. Validated for WB,IP,IHC,IHC,F. Available in 2 sizes. Highly specific and rigorously validated in-house, IFIT1 (D2X9Z) Rabbit Monoclonal Antibody (CST #14769) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:100 IHC Leica Bond: 1:400 - 1:1600 Immunohistochemistry (Paraffin): 1:800 - 1:3200 Flow Cytometry (Fixed/Permeabilized): 1:50 - 1:200 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 56086 . Protocol Available protocols: Western Blotting, Immunoprecipitation, IHC Leica Bond, Immunohistochemistry (Paraffin), Flow Cytometry (Fixed/Permeabilized) Specificity / Sensitivity IFIT1 (D2X9Z) Rabbit Monoclonal Antibody recognizes endogenous levels of total IFIT1 protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala194 of human IFIT1 protein. Background IFIT1 (interferon-induced protein with tetratricopeptide repeats 1) belongs to the IFIT family of proteins, which consists of four members in humans (IFIT1, IFIT2, IFIT3, and IFIT5) and three members in mice (IFIT1, IFIT2, and IFIT3) (1). IFIT1 expression is induced by type I interferons resulting from viral infection (2). IFIT1 is an antiviral protein that directly binds viral RNA that has a 5' triphosphate group (PPP-RNA) (2). In humans, the viral PPP-RNA bound IFIT1 forms a complex with IFIT2 and IFIT3 to sequester the viral PPP-RNA and prevent replication (2). IFIT1 has also been shown to inhibit translation by binding to the eukaryotic initiation factor 3 (eIF3) (3). Alternate Names C56; G10P1; GARG-16; IFI-56; IFI-56K; IFI56; IFIT-1; IFIT1; IFNAI1; interferon induced protein with tetratricopeptide repeats 1; Interferon-induced 56 kDa protein; interferon-induced protein 56; Interferon-induced protein with tetratricopeptide repeats 1; interferon-inducible mRNA 561; Interferon, alpha-inducible protein (MW 56kD); ISG56; P56; RNM561 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 56 Source/Isotype: Rabbit IgG