CST, 14775S, SB431542

Chemical Modulators for studying in the research area. Product Usage Information SB431542 is supplied as a lyophilized powder. For a 10 mM stock, reconstitute the 1 mg in 260.15 μl DMSO. Working concentrations and length of treatment can vary depending on the desired effect, but it is typically used as a pretreatment at 1-10 μM for 0.5-2 hr prior to treating with a stimulator. It can also be used alone, with varying treatment times lasting up to 24 hr. Storage Store lyophilized or in solution at -20ºC, desiccated. Protect from light. In lyophilized form, the chemical is stable for 24 months. Once in solution, use within 3 months to prevent loss of potency. Aliquot to avoid multiple freeze/thaw cycles. Background SB431542 is a potent and selective ATP-competitive inhibitor of the transforming growth factor β1 (TGF-β1) activin receptor-like kinases (ALK) -4, -5, and -7 (1-3). Research studies using cell-free kinase assays show that SB431542 inhibits ALK4 and ALK5 with IC values of 140 nM and 94 nM, respectively, and ALK7 with slightly less potency (2,3). The SB431542 inhibitor displays a 100-fold greater selectivity for ALK5 than 25 other kinases, including p38 MAPK and JNK1 (3). SB431542 inhibits Smad2 signaling induced by TGF-β and activin, but has no effect on BMP-induced Smad1 activation mediated by ALK -2, -3, and -6 (3,4). Additional studies show that SB431542 enhances the proliferation and integrity of ESC-derived endothelial cells (5). Alternate Names activin; ALK; SMAD; TFG; TGF-β1