CST, 14782T, CBS (D8F2P) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying CBS. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, CBS (D8F2P) Rabbit Monoclonal Antibody (CST #14782) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity CBS (D8F2P) Rabbit Monoclonal Antibody recognizes endogenous levels of total CBS protein. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu220 of human CBS protein. Background Cystathionine beta-synthase (CBS) is a key enzyme involved in sulfur amino acid metabolism because it catalyzes the formation of cystathionine from serine and homocysteine (1,2). The CBS protein contains a heme-binding domain that modulates enzyme activity by sensing redox changes or carbon monoxide binding (1). S-adenosylmethionine binds the carboxyl-terminal CBS domain to allosterically regulate CBS catalytic activity (3,4). In addition to catalyzing cystathionine formation, CBS also catalyzes the generation of hydrogen sulfide, a neuromodulator in the brain, through alternative reactions (5,6). Mutations in the corresponding gene result in homocystinuria, an autosomal recessive disorder characterized by abnormal sulfur metabolism, mental retardation, eye anomalies, and vascular disease (7). Alternate Names Beta-thionase; CBS; CBSL; Cystathionine beta-synthase; Cystathionine beta-synthase-like protein; cystathionine-beta-synthase; HIP4; methylcysteine synthase; Serine sulfhydrase Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 61 Source/Isotype: Rabbit IgG