CST, 14795S, DTX3L (D5F2J) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying DTX3L. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, DTX3L (D5F2J) Rabbit Monoclonal Antibody (CST #14795) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity DTX3L (D5F2J) Rabbit Monoclonal Antibody recognizes endogenous levels of total DTX3L protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val22 of human DTX3L protein. Background The E3 ubiquitin-protein ligase DTX3L (BBAP) is a deltex (DTX) family ligase that binds B aggressive lymphoma 1 (BAL1) to mediate the monoubiquitination of histone H4 Lys91 in response to DNA damage (1,2). The early recruitment of poly-ADP-ribose polymerase 1 (PARP1) protein to sites of DNA damage leads to poly-ADP-ribosylation (PARylation) of multiple target proteins. This results in the subsequent recruitment of DTX3L/BAL1 through binding of the BAL macrodomain to PARylated proteins. DTX3L/BAL1 then mediates the monoubiquitination of histone H4 Lys91 that is required for subsequent methylation of histone H4 Lys20 and recruitment of p53-binding protein 1 (1-3). DTX3L and BAL1 are highly expressed in malignant B-cell lymphomas and act to confer chemotherapy-resistance by protecting cells from DNA damaging agents (4). Alternate Names B-lymphoma- and BAL-associated protein; BBAP; deltex 3 like, E3 ubiquitin ligase; deltex 3-like; deltex 3-like (Drosophila); deltex E3 ubiquitin ligase 3L; DTX3L; E3 ubiquitin-protein ligase DTX3L; Protein deltex-3-like; rhysin 2; Rhysin-2; Rhysin2; RING-type E3 ubiquitin transferase DTX3L; RNF143 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 84 Source/Isotype: Rabbit IgG