CST, 14796T, Bax (D3R2M) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying Bax mouse. Validated for Western Blotting,Immunoprecipitation,Immunohistochemistry (Paraffin). Available in 2 sizes. Highly specific and rigorously validated in-house, Bax (D3R2M) Rabbit Monoclonal Antibody (CST #14796) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Immunohistochemistry (Paraffin): 1:400 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 28903 . Protocol Available protocols: Western Blotting, Immunoprecipitation, Immunohistochemistry (Paraffin) Specificity / Sensitivity Bax (D3R2M) Rabbit Monoclonal Antibody recognizes endogenous levels of total Bax protein in rodent samples. This antibody can detect human Bax protein by western blot, but not by other applications. Species Reactivity: Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu70 of mouse Bax protein. Background Bax is a key component for cellular induced apoptosis through mitochondrial stress (1). Upon apoptotic stimulation, Bax forms oligomers and translocates from the cytosol to the mitochondrial membrane (2). Through interactions with pore proteins on the mitochondrial membrane, Bax increases the membrane's permeability, which leads to the release of cytochrome c from mitochondria, activation of caspase-9, and initiation of the caspase activation pathway for apoptosis (3,4). Alternate Names Apoptosis regulator BAX; Bax; BCL2-associated X protein Specification REACTIVITY: M R SENSITIVITY: Endogenous MW (kDa): 20 Source/Isotype: Rabbit IgG