CST, 14798S, S6 Ribosomal Protein (5G10) Rabbit Monoclonal Antibody (Biotinylated)

Monoclonal Antibody for studying S6 Ribosomal Protein. Validated for Western Blotting. Highly specific and rigorously validated in-house, S6 Ribosomal Protein (5G10) Rabbit Monoclonal Antibody (Biotinylated) (CST #14798) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 140 mM NaCl, 3 mM KCI, 10 mM sodium phosphate (pH 7.4) dibasic, 2 mM potassium phosphate monobasic, 2 mg/mL BSA, and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity S6 Ribosomal Protein (5G10) Rabbit Monoclonal Antibody (Biotinylated) recognizes endogenous levels of total S6 ribosomal protein independent of phosphorylation. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues of human S6 ribosomal protein. Background One way that growth factors and mitogens effectively promote sustained cell growth and proliferation is by upregulating mRNA translation (1,2). Growth factors and mitogens induce the activation of p70 S6 kinase and the subsequent phosphorylation of S6 ribosomal protein. Phosphorylation of S6 ribosomal protein correlates with an increase in translation of mRNA transcripts that contain an oligopyrimidine tract in their 5' untranslated regions (2). These particular mRNA transcripts (5'TOP) encode proteins involved in cell cycle progression, as well as ribosomal proteins and elongation factors necessary for translation (2,3). Important S6 ribosomal protein phosphorylation sites include several residues (Ser235, Ser236, Ser240, and Ser244) located within a small, carboxy-terminal region of S6 protein (4,5). Alternate Names 40S ribosomal protein S6; Phosphoprotein NP33; ribosomal protein S6; RPS6; RS6; S6; S6 ribosomal protein; Small ribosomal subunit protein eS6 Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 32 Source/Isotype: Rabbit IgG