CST, 14810S, NUB1 Antibody

Polyclonal Antibody for studying NUB1L. Validated for Western Blotting. Highly specific and rigorously validated in-house, NUB1 Antibody (CST #14810) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity NUB1 Antibody recognizes endogenous levels of total NUB1 protein. Based upon sequence alignment, this antibody is predicted to detect both NUB1 and NUB1L. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human NUB1 protein. Antibodies are purified by protein A and peptide affinity chromatography. Background Both the NEDD8 ultimate buster 1 (NUB1) and the related NUB1L isoform are interferon-inducible adaptor proteins that negatively regulate ubiquitin-like protein NEDD8 (1,2). NUB1 protein contains an amino terminal ubiquitin-like (UBL) domain and multiple carboxy terminal ubiquitin-associated (UBA) domains. The NUB1L isoform is generated by alternative splicing and contains an extra UBA domain relative to NUB1 (2). Research studies indicate that NUB1 and NUB1L non-covalently bind NEDD8 and facilitate delivery of both NEDD8 monomers and NEDD8 conjugates to the proteasome for degradation (2-5). In addition, NUB1L binds and enhances the proteasomal degradation of the FAT10 ubiquitin-like protein (6). Additional research shows that NUB1 negatively regulates cell proliferation, likely due to inhibition of NEDD8 conjugation to SCF ubiquitin ligases, which leads to inhibition of p27 and cyclin E ubiquitination (3,7). NUB1 has been identified as a putative therapeutic target in Huntington's disease as NUB1 promotes a decrease in levels of mutant HTT protein (8). Alternate Names BS4; NEDD8 ultimate buster 1; NEDD8 ultimate buster-1; negative regulator of ubiquitin like proteins 1; Negative regulator of ubiquitin-like proteins 1; NUB1; NUB1L; NY-REN-18 antigen; NYREN18; Renal carcinoma antigen NY-REN-18 Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 78 SOURCE: Rabbit