CST, 14812S, PLK2 (D5R2B) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying PLK2. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, PLK2 (D5R2B) Rabbit Monoclonal Antibody (CST #14812) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:200 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity PLK2 (D5R2B) Rabbit Monoclonal Antibody recognizes endogenous levels of total PLK2 protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro67 of human PLK2 protein. Background At least five distinct polo-like kinases exist in mammalian cells: PLK1, PLK2, PLK3, PLK4/SAK, and the non-catalytic PLK5 protein (1). The p53-induced PLK2 functions in centriole duplication, as well as at spindle and S phase checkpoints (3-5). Research studies show that PLK2 expression is related to chemosensitivity in ovarian cancer. Downregulation of PLK2 expression in chemosensitive ovarian cancer cells is associated with a greater chance of relapse in patients following specific treatment regimens (6). PLK2 can phosphorylate α-synuclein at Ser129, which is a site shown to be involved in diseases of the central nervous system (7,8). Polo-like kinase 2 also phosphorylates GEFs and GAPs, regulating Ras and Rap small GTPase function in neurons (9). Alternate Names hPlk2; hSNK; PLK-2; PLK2; polo like kinase 2; Polo-like kinase 2; polo-like kinase 2 (Drosophila); Serine/threonine-protein kinase PLK2; Serine/threonine-protein kinase SNK; Serum-inducible kinase; SNK Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 70 Source/Isotype: Rabbit IgG